Mahdiyeh Ebrahim-nia, Amir Rashidlamir and Ali-akbar Hashemijavaheri
Ghrelin and obestatin are orexigenic and anorexigenic peptides, respectively, which are derived from preprogherlin and are believed to be important in the regulation of energy homeostasis and body weight control. The aim of this study was investigation of effect of a single session of circuit-resistance exercise and aerobic exercise on gene expression of obestatin in lymphocytes. In this study twenty-four trained female (age 20.91±3.11 yr, and body mass index 21.69±3.71 kg/m2 (mean±SE)) were randomly divided into two experimental groups and one control group. Experimental group 1 were asked to perform running a distance of 1.5 mile at a fixed speed (70% VO2max), experimental group 2 were asked to perform nonstop weight circuit exercises (9 exercises at 60%1-RM, 20s time for each, 3 circuits with 3min rest between circuits). Blood samples were collected before and immediately after exercise session. Lymphocyte separated and using lymphocyte density gradient centrifugation method and m-RNA purification performed by semi-quantitative PCR. Data's analyzed by one-way ANOVA test. The data indicated that although gene expression in experimental groups was increased a little, there was no significant increase in gene expression of obestatin in lymphocytes in these groups. In conclusion, It seems that it was due to the short length of training period and its intensity.
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