பரிசோதனை உயிரியல் ஐரோப்பிய இதழ் திறந்த அணுகல்

சுருக்கம்

Electrochemical Biosensor for Monitoring Insulin in Normal Individuals and Diabetic Mellitus Patients

Amandeep Kaur and Neelam Verma

A biosensor is an analytical tool that comprises two essential components – an immobilized biocomponent, in intimate contact with a transducer that converts a biological signal into a measurable electrical signal. In the present study, electrochemical insulin biosensor is described. It has been developed by immobilizing red blood cells (RBC) in carbon paste electrode (CPE) which contain enzyme hexokinase and glucose-6-phosphate dehydrogenase ,adeniosine triphosphate(ATP),and insulin receptor with concomitant generation of NADPH and its oxidation is measured amperiometrically at 0.32 V. The response is linear, covers the calibration range 0.006-0.09 nM with stability of two weeks and its detection limit is 0.006 nM which is significantly lower than the existing methods. The results were statistically significant with p value less than 0.0001. The conventional analytical techniques used although precise, are time consuming where as developed insulin biosensor have the advantage of ease of use, ecofriendly, sensitive, low cost and lower detection limit. The developed insulin biosensor has been applied for detection of insulin in normal subjects, non-insulin dependent and insulin dependent diabetes mellitus patients.

மறுப்பு: இந்த சுருக்கமானது செயற்கை நுண்ணறிவு கருவிகளைப் பயன்படுத்தி மொழிபெயர்க்கப்பட்டது மற்றும் இன்னும் மதிப்பாய்வு செய்யப்படவில்லை அல்லது சரிபார்க்கப்படவில்லை
இந்தப் பக்கத்தைப் பகிரவும்