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Inhibitory Role of Natural IgG Antibodies against VEGFR1 in the Proliferation of Pancreatic Cancer

Lifang Wu, Ziyi He, Yile Huang, Weiyi Cai, Qingyong Meng, Jun Wei

Context Our recent study suggested that natural IgG antibodies against vascular endothelial growth factor receptor 1 (VEGFR1) had a role in inhibiting the proliferation of nasopharyngeal carcinoma (NPC) cells. Objective The present work was designed to test if plasma anti- VEGFR1 IgG could also inhibit the proliferation of pancreatic cancer cells. Design PANC-1 and SW1990 cell lines derived from pancreatic cancer were used for this in vitro study. They were cultured with 15% human plasma either positive or negative for anti-VEGFR1 IgG. Main outcome measures Cell viability was detected by cell counting kit-8. Flow cytometry was used to analyse cell apoptosis and quantitative real-time PCR was used to examine the expression of VEGFR1 mRNA. Setting Guangdong Medical University, Dongguan, China. Results Plasma anti-VEGFR1 IgG exhibited significant inhibitory effects on the proliferation of PANC-1 cell line but not SW1990 cell line; the proportion of apoptotic cells was higher in PANC-1 cells treated with anti-VEGFR1 IgG positive plasma than those cells treated with anti- VEGFR1 IgG negative plasma. Moreover, the expression of VEGFR1 mRNA was significantly higher in PANC-1 cells than SW1990 cells. Conclusions Plasma anti-VEGFR1 IgG antibody may be a promising agent for the treatment of pancreatic cancer that highly expresses VEGFR1.